Figure 1.

Combined time-point analysis of scRNA sequencing shows distinct TdTomato-expressing clusters

(A) Experimental schematic showing injury model and cells harvested in the uninjured limb (n = 1), 1 week following BT injury (n = 1), and 6 weeks following BT injury (n = 2) from Hoxa11-CreER^T2:ROSA-TdTomato mice that were processed and sequenced. Mouse schematic adapted from our group's previous work (Agarwal et al., 2016a).

(B) UMAP cluster, cluster definitions, and cell counts of combined time-point analysis.

(C) Violin plots of combined time-point and individual time-point TdTomato expression.

(D) Violin plots of gene markers for mesenchymal and bone progenitors.