Figure 2.

Trajectory analysis of TdTomato⁺ cells shows osteo/chondro branch following traumatic injury

(A) Monocle 2 pseudo-time analysis separated by TdTomato-expressing cluster.

(B) Feature plots of Pdgfra and Clec3b gene expression levels.

(C) Feature plots of chondrocyte differentiation genes Acan and Sox9 expression levels.

(D) Feature plots of osteoblast differentiation genes Runx2 and Spp1 expression levels.

(E) Feature plots of tendon differentiation genes Scx, Tnmd, and Comp expression levels.

(F) Pseudo-time plots separated by time point with osteo/chondro branch circled in orange.

(G) UMAP steam plot of RNA velocity of TdTomato⁺ cells in clusters MSC:0, MSC:2, MSC:14, and Teno:16 (left) with arrows showing velocity and UMAP latent time plot (right).

(H) CytoTrace analysis of TdTomato-expressing cells including a UMAP plot with predicted ordering score (left), a UMAP plot colored by cluster (center), and a box plot showing predicted score of clusters (right).