Figure 1.

Morphological Improvements with Maturation Methods in CUSO-2 hiPSC-CMs

(A) Representative images of staining for various sarcomeric proteins in hiPSC-CMs cultured under each condition. cTNI, cardiac troponin I; MLC-2V, myosin light chain, ventricular isoform; MYBPC3, myosin-binding protein C3.

(B) Average cell areas in hiPSC-CMs cultured under each condition.

(C) Cellular circularity, defined by circularity = 4 × π × area/perimeter² in hiPSC-CMs cultured under each condition. A lower circularity is indicative of more elongated objects. Data were pooled from three replicates from two inductions (GLPAT) or three or more (all other conditions) inductions, shown as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; Kruskal-Wallis test followed by Dunn's post test (B) or one-way ANOVA with Tukey's post test (C).

See also Figure S1.