Figure 3.

RBBP4 shares target genes with PRC2

(A) Pie chart showing the distribution of RBBP4 binding sites in mouse ESCs.

(B) GO enrichment analyses for genes that RBBP4 binds to. The biological processes of the top 20 are shown.

(C) Venn diagram showing the overlap between genes differentially expressed after Rbbp4 deletion and those occupied by RBBP4. GO analysis for biological processes associated with the overlapping genes.

(D) Venn diagram showing the overlap between genes that RBBP4 binds to and those occupied by SUZ12, EED, or H3K27me3.

(E) GO analysis of overlapping genes between RBBP4 binds and PRC2 targets.

(F) Genome browser tracks to show RBBP4 occupancy near lineage-specific markers for trophectoderm (Cdx2), mesoderm (Brachyury, Tbx2), and endoderm (Sox17, Gata4), Oct4 and Sox2 (up). ChIP-qPCR data showing binding of RBBP4-FLAG to representative RBBP4 target promoters (bottom).

Data are plotted as mean ± SD. (n = 3). ^∗p < 0.05, ^∗∗p < 0.01. See also Figure S4.