Figure 4.
Rbbp4 knockout significantly reduces the expression of pluripotency-associated genes and promotes mesendodermal gene expression in ESCs
(A) Time-course analyses of pluripotency-associated genes and the lineage-specific markers for trophectoderm, mesoderm, and endoderm expression levels in Rbbp4F/F ESCs after transient expression of Cre recombinase. Expression is normalized by β-actin.
(B) Immunofluorescence showing co-staining of the pluripotency markers (OCT4, SOX2, and NANOG) and the lineage-specific markers (FOXA2, GATA6, and CDX2) at the indicated time points after expressing Cre recombinase in Rbbp4F/F ESCs, respectively. DAPI (blue), pluripotency marker genes (green), the lineage-specific markers (red), and the merge picture are shown. Magnification, 63×.
Data are represented as mean ± SD of three independent experiments.