Skip to main content
. 2021 Feb 25;16(3):582–596. doi: 10.1016/j.stemcr.2021.01.017

Figure 1.

Figure 1

LIF Alone Supports ESC Self-Renewal and Pluripotency

(A) Experimental outline of the L-ESC derivation procedure from 2i/L-ESCs.

(B) 2i/L-ESCs were switched to L medium and cultured to passages 3 (P3), P5, and P25. Here we used 2i/L-ESCs with the GOF/GFP reporter. Scale bars, 100 μm. See also Figure S1A.

(C) Karyotyping of L-ESCs (P30, n = 50, results of three independent experiments).

(D) Immunostaining of OCT4, SOX2, and NANOG in L-ESCs (results of three independent experiments). Scale bars, 50 μm.

(E) Single-cell clonogenicity efficiency in L-ESCs and 2i/L-ESCs (n = 480 single cells of L-ESCs and 2i/L-ESCs, respectively; results of three independent experiments).

(F) L-ESCs were treated with JAK inhibitor I after day 3 P2 and day 10 P4 (results of three independent experiments). Scale bars, 100 μm.

(G) 2i/L-ESCs were treated with JAK inhibitor I after day 3 P2, day 10 P6, and day 16 P10 (results of three independent experiments). Scale bars, 100 μm.