Figure 3.

COL10A1 and MATN3 mutants retain COL10 or MATN3 within the ER

(A and B) Immunostaining of COL10 (A) or MATN3 (B) and PDI on day 56 of HI. Arrowheads indicate intracellular aggregates in mutants. Similar results were obtained in n = 4 independent experiments. Scale bars, 100 μm.

(C and D) Intracellular retention quantified from fluorescence intensity of COL10 (C) or MATN3 (D) co-staining with PDI (intracellular) divided by total fluorescence intensity in n = 4 independent experiments on day 56 of HI. Values are expressed as mean ± SEM. (n.s., no significant difference; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001 by unpaired two-sided t test).

See also Figure S5.