Figure 5.

COL10A1 and MATN3 mutants show zonal disorganization without change in mineralization

(A and B) Histology of growth-plate-like structures from tissue collected on day 56 after transplantation into immunodeficient mice. (Top) Safranin O staining; (bottom) von Kossa staining. Similar results were observed in two or more growth-plate-like structures for each clone. Scale bars, 200 μm.

(C and D) Immunofluorescence, immunohistochemistry, and TUNEL staining of the growth-plate-like structures in (A) and (B). Scale bars, 200 μm.

(E) von Kossa staining of mutant (top) or isogenic control (bottom) pellets transplanted on day 28 of HI and collected after 20 days in vivo. Similar results were obtained in six pellets transplanted into three different mice (two pellets per mouse). Scale bars, 1 mm.

(F) Quantification of von Kossa-positive areas. Values are expressed as mean ± SEM. Results are calculated as n = 3 from three different mice with two pellets each. (n.s., no significant difference by unpaired two-sided t test).