Fig. 1.
Evaluation of maximum achievable myocardial TRPM4 protein knockdown in cardiomyocyte-specific TRPM4 knockout mice. a Course of tamoxifen treatment in TRPM4fx/fx/αMHC-CreERT2-postive and TRPM4fx/fx/αMHC-CreERT2-Cre negative mice. b Western blot analysis with anti-TRPM4 and anti-Calnexin 2 weeks after tamoxifen injections. c Quantification of the relative TRPM4 protein level in the heart of TRPM4fx/fx/αMHC-CreERT2-positive (Cre+) mice in comparison to TRPM4fx/fx/αMHC-CreERT2-negative (Cre−) mice. Relative protein level of TRPM4 is expressed as mean ± SD. ***p <0.005