Fig. 1.

miR-126-3p overexpression followed by pSILAC in 3T3-L1 pre-adipocytes to determine the general proteomic profile. (a) 3T3-L1 pre-adipocytes were grown in supplemented DMEM high-glucose media until they reached 60–70% confluence. From this point, (b) cells were transfected with scrambled (CTL) or miR-126-3p mimic for 8 h. (c) Later, normal DMEM media was replaced with SILAC DMEM containing medium lysine (M; for CTL) or heavy lysine (H; for miR-126-3p overexpression) for 24 h, the time required for 3T3-L1 cell turnover and incorporation of the respective amino acid. (d) CTL or miR-126-3p-mimic-treated cell protein extracts were combined at the same proportion (based on protein quantification) and (e) differences in protein expression were detected by quantitative mass spectrometry