Fig. 2.

Protein general profile after miR-126-3p overexpression. (a) miR-126-3p expression normalised by U6 housekeeping mRNA, n = 3 per group. (b) Scatter plot representing the general proteomic profile found for miR-126-3p-mimic-transfected 3T3-L1 pre-adipocytes. The normalised heavy (miR-126-3p)/medium (scrambled; CTL) ratio was used as the basis of the graph. (c) IRS-1 protein expression normalised by α-tubulin, n = 3 per group. Data were analysed by unpaired Student’s t test (a, c, CTL vs miR-126 mimic); *p < 0.05 and **p < 0.01. All the graphs represent mean ± SEM. Downreg., downregulated