Fig. 3.

miR-126-3p directly regulates Lunapark protein expression. (a) Lunapark 3′UTR and its respective region of interaction within mmu-miR-126-3p sequence (miRNA seed sequence presented in red characters). (b) Representative blots and graphical representation of Lunapark protein expression normalised to α-tubulin, n = 3 per group. (c) Lnpk mRNA expression normalised by cyclophilin, n = 3 per group. (d) Relative firefly luciferase normalised by renilla luciferase activity measured in cells transfected with either a pmiRGlo vector with no insert or a pmiRGlo vector containing Lnpk-3’ UTR sequence, n = 12 per group. Data were analysed by unpaired Student’s t test (b, c, scrambled [CTL] vs miR-126 mimic; d, CTL vs miR-126 mimic, with or without siRNA targeting Lnpk); *p < 0.05 and ***p < 0.001. All the graphs represent mean ± SEM