Fig. 5.

Investigation of myogenic regulatory factors (MRFs) and muscle specific proteins in C2C12 during myogenesis. (a) Real-time q-PCR showing the transcripts levels of early and late MRFs; data are representative of three experiments and expressed as means ± SD. (b) Western blotting shows a similar expression of MyoD in undifferentiated myoblasts and in the early stage of C2C12 differentiation (T0 and T1), while it decreased in the intermediate and late stages (T3–T10). On the opposite MyHC-1 starts to be expressed from the intermediate to late stage of differentiation. (c) The bands were quantitated by calculating the relative quantities of MyoD and MyHC-1 normalized to Actin; data are representative of three experiments