Table 2.
List of genes and primer sequences used for RT-PCR
| Genes | NCBI RefSeq | Primer pairs sequence (5′->3′) | RT-PCR product size (base pair) |
|---|---|---|---|
| Tnpo3 | NM_177296.4 |
GAGTTTCGAATGAGAGTGTC CAGCCATGATAAAGAGAACC |
145 |
| MyoD | NM_010866.2 |
GCTTAAATGACACTCTTCCC AGGACTACAACAACAACAAC |
131 |
| Myf5 | NM_008656.5 |
AGGTGGAGAACTATTACAGC TGATACATCAGGACAGTAGATG |
152 |
| Desmin | NM_010043.2 |
ACACCTAAAGGATGAGATGG GAGAAGGTCTGGATAGGAAG |
147 |
| Pax7 | AF254422.4 |
GTATAAGAGAGAGAACCCCG GCCATCTTCTTCTTTCTTGTC |
175 |
| MyoG | NM_031189.2 |
AGTACATTGAGCGCCTAC CAAATGATCTCCTGGGTTG |
182 |
| Myf6 | NM_008657.2 |
ATAACTGCTAAGGAAGGAGG AAGAATGTTCCAAATGCTGG |
160 |
| GAPDH | NM_001256799.2 |
CTCTGATTTGGTCGTATTGG GTAAACCATGTAGTTGAGGTC |
111 |