Table 3.

Composition of buffers used for protein extraction from nuclear and cytoplasmic fractions

Type of buffer	Composition	Company
RIPA	25 mM Tris-HCl pH 7.5	Sigma-Aldrich, St.Louis, Missouri, USA
	50 mM NaCl2
	0.5% Na-deoxycholate	Thermo Fisher Scientific, Waltham, Massachusetts, USA
	1% NP-40	Sigma-Aldrich, St.Louis, Missouri, USA
	0.1% SDS
Hypotonic lysis buffer	10 mM HEPES pH 7.9	All from Sigma-Aldrich, St.Louis, Missouri, USA Missouri, USA
	1.5 mM MgCl2
	10 mM KCl
	completeTM EDTA-Free 2×	Roche, Basle, Switzerland
S100 buffer	0.3 M HEPES pH 7.9	All from Sigma-Aldrich, St.Louis, Missouri, USA
	30 mM MgCl2
	1.4 mM KCl
	completeTM EDTA-Free 2×	Roche, Basle, Switzerland
Low salt buffer	20 mM HEPES pH 7.9	Sigma-Aldrich, St.Louis, Missouri, USA
	25% glycerol	Thermo Fisher Scientific, Massachusetts, USA
	20 mM KCl	All from Sigma-Aldrich, St.Louis, Missouri, USA
	1.5 mM MgCl2
	0.2 mM EDTA
	completeTM EDTA-Free 2×	Roche, Basle, Switzerland
High salt buffer	20 mM HEPES pH 7.9	Sigma-Aldrich, St.Louis, Missouri, USA
	25% glycerol	Thermo Fisher Scientific, Massachusetts, USA
	1.2 M KCl	Sigma-Aldrich, St.Louis, Missouri, USA
	1.5 mM MgCl2
	completeTM EDTA-Free 2×	Roche, Basle, Switzerland