Table 4.
List of primary and secondary antibodies and dilutions used for WB, IF and IEM analyses
| Dilution | Company | |
|---|---|---|
| Primary antibodies | ||
| Skeletal Muscle Myosin (F59) |
For WB: 1/200 For IF: 1/200 |
Santa Cruz Biotechnology, Dallas, Texas, USA |
| Myogenin (5FD) |
For WB: 1/200 For IF: 1/200 |
|
| MyoD (G-1) | For WB: 1/200 | |
| TNPO3 (ab71388) |
For WB: 1/1,000 For IF: 1/200 For IEM: 1/20 |
Abcam, Cambridge, UK |
| SRSF1 (96) |
For WB: 1/250 For IF: 1/100 |
Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| Actin (I-19) | For WB:1/500 | Santa Cruz Biotechnology, Dallas, Texas, USA |
| Secondary antibodies | ||
| Goat Anti-Mouse IgG (H + L), DyLight 488 | For IF: 1/1,000 | Thermo Fisher Scientific, Waltham, Massachusetts, USA |
| Goat Anti-Rabbit IgG (H + L), DyLight 650 | For IF: 1/250 | |
| Amersham ECL Anti-mouse IgG HRP-conjugated | For WB: 1/1,000 | GE Healthcare, Chicago, Illinois, USA |
| Amersham ECL Anti-rabbit IgG HRP-conjugated | For WB: 1/1,000 | |
| Anti-goat IgG HRP-conjugated | For WB: 1/10,000 | Jackson ImmunoResearch, Cambridge, UK |
| Goat anti-rabbit conjugated with 10 nm colloidal gold particles | For IEM 1/20 | BBInternational, Cardiff, UK |