Table 5.
TNPO3 fluorescence intensity (gray levels ± SD) | ||||
---|---|---|---|---|
Undifferentiated myoblasts | Differentiated myotubes | |||
(Nucleus) | (Cytoplasm) | (Nucleus) | (Cytoplasm) | |
T0 | 354 ± 59 | 173 ± 81 | – | – |
T1 | 525 ± 73 | 205 ± 54 | – | – |
T5 | 38 ± 25 | 8 ± 7 | 259 ± 58 | 201 ± 18 |
T10 | 42 ± 38 | 10 ± 6 | 452 ± 27 | 294 ± 60 |
Fluorescence intensity relative to TNPO3 in both nuclear and cytoplasmic compartments of undifferentiated myoblasts and differentiated myotubes. The fluorescence intensity, as differentiation proceeded, increased in differentiated myotubes, particularly in nuclear domain. Data are representative of three experiments and expressed as mean ± SD