Table 6.

Diagnoses and results of bacterial culture, sequencing, fluorescence in situ hybridization (FISH), and histology for eight bovine abortion cases that were FISH-screened for T. pyogenes lesion association.

Case ID	Culture T. pyogenes ≥1 organ	Sequencing >10,000T. pyogenes reads placentaa	Sequencing T. pyogenes in extraction control	Sequencing T. pyogenes among three most abundant placenta	Sequencing T. pyogenes among three most abundant lung–liver	Sequencing most abundant placenta	Sequencing most abundant lung–liver	FISH T. pyogenes	FISH T. pyogenes lesion-associated	Histological findings	Previous etiology	Final etiology
Case 6	+	+	–	+	+	T. pyogenes	T. pyogenes	+	+	Suppurative bronchopneumonia	T. pyogenes	T. pyogenes
Case 28	–	+	–	+	+	Bacteroides unclassified	Lactococcus unclassified	+	+	Necrosuppurative placentitis Suppurative bronchopneumonia	Unknown	T. pyogenes
Case 33	–	+	–	+	+	T. pyogenes	Enterococcus durans	+	+	Necrosuppurative placentitis Suppurative bronchopneumonia Non-suppurative encephalitis	Unknown	T. pyogenes
Case 40	+	–	+	NA	–	NA	Acinetobacter unclassified	+	+	Suppurative bronchopneumonia	T. pyogenes	T. pyogenes
Case 41	+	+	–	+	+	T. pyogenes	T. pyogenes	+	+	Suppurative placentitis Suppurative bronchopneumonia	T. pyogenes	T. pyogenes
Case 42	+	–	–	NA	–	NA	Bacteroides fragilis	+	+	Suppurative bronchopneumonia	T. pyogenes	T. pyogenes
Case 43	+	+	–	+	+	Staphylococcus vitulinus	T. pyogenes	+	+	Necrosuppurative placentitis Suppurative bronchopneumonia	T. pyogenes	T. pyogenes
Case 44	–	+	–	NA	+	NA	T. pyogenes	+	+	Suppurative bronchopneumonia BVDV antigen in tissue	BVDV	BVDV, T. pyogenes

–, no/negative; +, yes/positive; BVDV, bovine viral diarrhea virus; NA, data not available.

^aIf placenta was not available, the number of reads from the lung–liver pool or lung was assessed.