Table 7.
Comparison of diagnoses made using routine methods (20) with the diagnoses reached by combining routine methods with a 16S rDNA amplicon sequencing–guided FISH analysis.
| Diagnostic group | No. of cases routine methods | % of total routine | No. of cases combined methods | % of total combined |
|---|---|---|---|---|
| 1. Likely cause of abortion identified | 53a | 46 | 61a | 53 |
| N. caninumb | 31 | 27 | 31 | 27 |
| T. pyogenes | 5 | 4 | 7 | 6 |
| E. coli | 4 | 4 | 6 | 5 |
| E. coli, B. licheniformis | 0 | 0 | 1 | <1 |
| S. aureus | 3 | 3 | 3 | 3 |
| L. monocytogenes | 2 | 2 | 2 | 2 |
| B. licheniformis | 1 | <1 | 3 | 3 |
| Other bacterial speciesc | 4 | 4 | 4 | 4 |
| Fungi | 2 | 2 | 3 | 3 |
| BVDV | 1 | <1 | 0 | 0 |
| BVDV, T. pyogenes | 0 | 0 | 1 | <1 |
| 2. Lesions present, specific etiology not identified | 62a | 54 | 54a | 47 |
| Suppurative to necrosuppurative placentitis and suppurative bronchopneumonia | 12 | 10 | 8 | 7 |
| Suppurative to necrosuppurative placentitis | 26 | 23 | 21 | 18 |
| Suppurative bronchopneumonia | 14 | 12 | 15 | 13 |
| Non-suppurative placentitis | 8 | 7 | 8 | 7 |
| Interstitial pneumonia | 1 | <1 | 1 | <1 |
| Granulomatous pneumonia | 1 | <1 | 1 | <1 |
| Total | 115 | 100 | 115 | 100 |
BVDV, bovine viral diarrhea virus.
a
Accumulated figures within the respective diagnostic group.
b
In two of these cases, E. coli and L. garvieae were identified as coinfecting agents, respectively.
c
Streptococcus sp., Klebsiella pneumoniae, Aeromonas sp., and Lactococcus garvieae (one case each).
Etiologic diagnoses for 115 bovine abortion cases with lesions suggestive for infection out of 162 total cases.