TABLE 1.

Selectable marker genes used in Aspergillus oryzae.

Selectable marker gene	Description	References
niaD	The niaD gene encodes a nitrate reductase, and the niaD– mutants display chlorate resistance	Unkles et al., 1989
pyrG	The pyrG gene encodes an orotidine-5′-phosphate (OMP) decarboxylase, and pyrG-deficient strains can be positively selected by 5-fluoroorotic acid (5-FOA)	Mattern et al., 1987
argB	The argB gene encodes an ornithine carbamoyltransferase and allows the complementation of the arginine auxotrophic mutation	Gomi et al., 1987
sC	sC marker gene encodes an ATP sulfurylase, and the sC– mutants can be isolated by positive selection for selenate resistance	Yamada et al., 1997
adeA	The adeA gene encodes a phosphoribosylaminoimidazolesuccinocarboxamide synthase of purine biosynthetic pathway and could complement the adenine auxotrophic mutants	Jin et al., 2004a
adeB	The adeB gene encodes a phosphoribosylaminoimidazole carboxylase of purine biosynthetic pathway and could complement the adenine auxotrophic mutants	Jin et al., 2004a
amdS	The amdS gene encodes an acetamidase that is required for acetamide utilization	Gomi et al., 1991
ptrA	The ptrA is a pyrithiamine-resistant gene that was cloned from a pyrithiamine resistant mutant of A. oryzae	Kubodera et al., 2000
thiI	The thiI gene encodes a thiamine transporter gene, and its mutant showed pyrithiamine resistance	Todokoro et al., 2020
blmB	The blmB gene encodes bleomycin N-acetyltransferase from bleomycin-producing Streptomyces verticillus and is responsible for the bleomycin resistance	Suzuki et al., 2009
AosdhB(cxr)	AosdhB encodes a succinate dehydrogenase gene, and its mutation [AosdhB(cxr)] confers the carboxin resistance.	Shima et al., 2009