TABLE 2.
Promoters used for the production of recombinant proteins in Aspergillus oryzae.
| Promoter gene | Species | Description | Produced recombinant protein | References |
| amyB | Aspergillus oryzae | An alpha-amylase gene (amyB) promoter. | A mature human lysozyme (HLY); | Tsuchiya et al., 1992 |
| Recombinant human lactoferrin | Ward et al., 1992 | |||
| Thielavia terrestris glucoamylase | Rey et al., 2003 | |||
| Pycnoporus coccineus extracellular laccase | Hoshida et al., 2005 | |||
| Aspergillus fumigatus elastase inhibitor AFUEI | Yamashita et al., 2011 | |||
| A novel fungal α-amylase (PcAmy) gene from Penicillium sp. | Yin et al., 2015 | |||
| Leucine aminopeptidase A (lapA) from A. oryzae | Matsushita-Morita et al., 2011 | |||
| glaA142 | A. oryzae | An improved glucoamylase gene promoter PglaA142 | Candida antarctica lipase B (CALB) | Tamalampudi et al., 2007 |
| Mite allergen Der f 7 | Tokuoka et al., 2008 | |||
| A. oryzae enzymes (BglA, BglF, and BglJ) | Kudo et al., 2015 | |||
| A. oryzae recombinant tannase (AotanB) | Ichikawa et al., 2020 | |||
| glaB | A. oryzae | A glucoamylase-encoding gene (glaB) promoter for recombinant protein production in solid-state fermentation of A. oryzae | A. oryzae glucoamylase A (glaA) and glucoamylase B (glaB) | Ishida et al., 2006 |
| tef1 | A. oryzae | Promoter of a translation-elongation factor 1 alpha gene, tef1 | Polygalacturonase (pgaA and pgaB) | Kitamoto et al., 1998 |
| melO | A. oryzae | The tyrosinase-encoding gene (melO) promoter | E. coli beta-glucuronidase (GUS) | Ishida et al., 2001 |
| gpdA | Aspergillus nidulans | The constitutive gpdA promoter from A. nidulans | Bacterial beta-galactosidase (lacZ) and beta-glucuronidase (uidA) | de Ruiter-Jacobs et al., 1989 |
| GFP and DsRed fluorescent proteins | Nguyen et al., 2016 | |||
| Seven polyketide synthase (PKS) genes from Xylaria sp. | Punya et al., 2013 | |||
| Hemoglobin domains (HBD) isolated from A. oryzae and Aspergillus niger | te Biesebeke et al., 2006 | |||
| thiA | A. oryzae | The thiamine-regulatable promoter of the thiamine thiazole synthase gene (PthiA) | Enhanced green fluorescent protein (EGFP) | Shoji et al., 2005 |
| enoA142 | A. oryzae | An improved enolase promoter (P-enoA142) | Fusarium heterosporum lipase (FHL) | Takaya et al., 2011 |
| An improved enoA promoter that harbored 12 tandem repeats of the cis-acting element (region III) of A. oryzae | Beta-glucuronidase (GUS) | Tsuboi et al., 2005 | ||
| kojA | A. oryzae | A promoter of the secondary metabolism gene kojA involved in the kojic acid biosynthesis, for the production of other secondary metabolites by A. oryzae | A polyketide synthase (wA) of A. oryzae | Tamano et al., 2019 |
| hlyA | A. oryzae | A hemolysin-like protein encoding gene (hlyA) promoter used for protein overexpression in A. oryzae grown in solid-state culture | A. oryzae endo-1,4-β-glucanase (CelA) and endoglucanase CelB; Trichoderma reesei endoglucanases (TrEglI and TrEglIII) | Bando et al., 2011 |
| actB | A. oryzae | Promoter of an actin-encoding actB gene that can be highly induced by benomyl treatment | Antifungal compounds | Marui et al., 2010b |
| pgkA | A. oryzae | A constitutive phosphoglycerate kinase (PGK) gene promoter | PEP carboxylase (PPC) or PEP carboxykinase (PCK) genes from E. coli | Liu et al., 2017 |
| sodM | A. oryzae | Promoter of manganese superoxide dismutase-encoding gene sodM | Beta-glucuronidase (GUS) | Ishida et al., 2004 |
| 6-phosphofructokinase (pfk) | Liu et al., 2017 |