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. 2021 Feb 23;12:595140. doi: 10.3389/fimmu.2021.595140

Figure 2.

Figure 2

SFTSV infection triggers NLRP3 inflammasome activation and IL-1β production and secretion. (A, B) THP-1 macrophages were treated with LPS (1 μg/ml) for 4 h (or then 2 μM Nigericin for 30 min) or infected with SFTSV for 48 h at different MOIs (0.1, 0.5, 1, or 2). (C, D) THP-1 macrophages were treated with LPS (1 μg/ml) for 4 h (or then 2 μM Nigericin for 30 min) or infected with SFTSV at an MOI = 1 for 24, 36, 48, or 60 h. (A, C) IL-1β levels in the supernatant were determined by ELISA. (B, D) Mature IL-1β and cleaved caspase-1 in supernatants or NLRP3, pro-caspase-1, cleaved caspase-1 pro-IL-1β, ASC, SFTSV NP and β-actin in lysates were determined by Western blot. (E) THP-1 macrophages were treated with LPS (1 μg/ml) for 4 h (or then 2 μM Nigericin for 30 min) or infected with SFTSV at an MOI = 1 for 48 h. ASC oligomerization was determined by Western blot. Data are mean values ± SD derived from the samples collected in triplicate. ***P<0.001.