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. 2021 Feb 23;12:595140. doi: 10.3389/fimmu.2021.595140

Figure 3.

Figure 3

SFTSV infection induced IL-1β secretion is dependent on active caspase-1 and NLRP3 assembly. (A, B) THP-1 macrophages were treated with the caspase-1 inhibitor (VX-765) at indicated concentration for 1 h prior to infection. Then the cells were treated with LPS (1 μg/ml) for 4 h (or then 2 μM Nigericin for 30 min) or infected with SFTSV at an MOI = 1 for 48 h. (C, D) THP-1 macrophages were treated with the NLRP3 assembly inhibitor (CY-09) at indicated concentration for 1 h prior to infection. Then the cells were treated with LPS (1 μg/ml) for 4 h (then 2 μM Nigericin for 30 min) or infected with SFTSV at an MOI = 1 for 48 h. (A, C) IL-1β levels in the supernatant were determined by ELISA. (B, D) Mature IL-1β and cleaved caspase-1 in supernatants or NLRP3, pro-caspase-1, cleaved caspase-1 pro-IL-1β, ASC, SFTSV NP and β-actin in lysates were determined by Western blot. Data are mean values ± SD derived from samples collected in triplicate. ***P<0.001.