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. 2021 Feb 23;12:595140. doi: 10.3389/fimmu.2021.595140

Figure 4.

Figure 4

The role of NLRP3 inflammasome in the regulation of SFTSV induced IL-1β secretion. (A) THP-1 cells expressing shRNAs with the target at ASC, NLRP3, or pro-caspase-1 were lysed, and the targeted proteins were determined by Western blot at 3 days after lentiviral particles infection. THP-1 cells expressing shRNAs targeting ASC, NLRP3, or pro-caspase-1 were infected with SFTSV (MOI = 1) or mock infection for 48 h. (B) IL-1β levels in the supernatant were determined by ELISA. (C, F) Mature IL-1β and cleaved caspase-1 in supernatants or NLRP3, pro-caspase-1, cleaved caspase-1 pro-IL-1β, ASC, SFTSV NP and β-actin in lysates were determined by Western blot. (D) THP-1 cells (wild type and NLRP3 KO) were lysed, and the targeted proteins were determined by Western blot. (E) Differentiated THP-1 cells (wild type and NLRP3 KO) were infected with SFTSV (MOI = 1) or mock infection for 24 or 48 h. SFTSV viral RNA and β-actin mRNAs were quantified by qPCR. (G–I) Differentiated THP-1 cells (wild type and NLRP3 KO) were infected with SFTSV (MOI = 1) or treated with LPS/Nigericin or mock infection for 48 h. IL-1β, TNF-α and IL-6 levels in the supernatant were determined by ELISA. Data are mean values ± SD derived from the samples collected in triplicate. ***P < 0.001, NS, no significance.