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. 2021 Mar 8;4:310. doi: 10.1038/s42003-021-01790-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The bispecific LiTE molecule (49 kDa) containing an anti-EGFR nanobody (green) linked to anti-CD3 scFv (blue). HSA (red) and point mutationed variants (yellow dots) with different affinities for human FcRn were genetically fused with the LiTE and expressed as a single polypeptide (108 kDa).