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. 2021 Mar 8;4:310. doi: 10.1038/s42003-021-01790-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Protein was i.v. injected in 4 groups of mice (N = 7 per group) and blood samples were drawn at 8 time points. Full size construct was detected in serum by ELISA. The β half-life and the R² of the fit to the curve for each construct are listed for LiTE (triangle), Albu-LiTE-NB (diamond), Albu-LiTE-WT (square) and Albu-LiTE-HB (circle). Data was analysed with Graphpad prism v.8 using two-phase decay for calculating the molecular half-life (T_½ β) and statistical differences between the constructs, detected in serum over time using a two-way ANOVA with multiple comparisons and Tukey’s post hoc correction. **p < 0.01. Error bars represent standard deviations.