Table 1.
Human FcRn binding affinities for recombinant human serum albumin (HSA) variants and Albu-LiTE constructs.
| Construct | KD (M) | kon (1/Ms) | kdis (1/s) | R2 1:1 fit | χ2 1:1 fit |
|---|---|---|---|---|---|
| NB HSA | – | – | – | n.s. | – |
| Albu-LiTE-NB | – | – | – | n.s. | – |
| WT HSA | 7.5E-07 | 1.3E+04 | 9.7E-03 | 0.995 | 0.029 |
| Albu-LiTE-WT | 1.1E-08 | 4.1E+04 | 4.7E-04 | 0.993 | 0.161 |
| HB HSA | 5.8E-09 | 4.0E+04 | 2.3E-04 | 0.999 | 0.058 |
| Albu-LiTE-HB | 2.9E-09 | 3.6E+04 | 1.0E-04 | 0.999 | 0.073 |
Measured binding affinities (KD, kon and kdis) for HSA and Albu-LiTE constructs with χ2 and R2 for the fits are shown for each construct. Binding affinities could not be determined for the Null binding (NB) HSA and Albu-LiTE-NB construct due to low binding responses and lack of fit in a 1:1 binding model, n.s. non-significant.