Figure 1.

Characterization of the Differentiation Status of Human Enteroids

(A–C) (A) Fold change of expression levels of cell-type markers in the organoids cultured in different differentiation media (H10, H5, and H0) versus those cultured in expansion medium (Ex). Data show the mean and SD of three independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 analyzed by Student's t test, two tailed. The percentages of enterocytes (B) and Paneth cells (C) in the organoids cultured in different differentiation media and expansion medium. The representative histograms of one experiment (left), and means and SD of three independent experiments (right) are presented.

(D) Confocal images of VIL1⁺ enterocytes, MUC2⁺ goblet cells, LYZ⁺ Paneth cells, and CHGA⁺ enteroendocrine cells in the organoids cultured in H0 medium. Nuclei and cellular actin filaments are counterstained with DAPI (blue) and Phalloidin-647 (purple). Scale bar, 10 μm.

See also Figure S1.