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. 2021 Feb 23;11:615568. doi: 10.3389/fonc.2021.615568

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Copyright © 2021 Liu, Liu, Yan, Liu, Fang and Fan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Huaier extract inhibited AR-FL and AR-V7 mRNA levels via targeting SMYD3 signaling. The mRNA levels of SMYD3 were measured by RT-qPCR in LNCaP and 22Rv1 time-(A) or dose-(B) dependently treated with huaier extract. The AR-FL and AR-V7 transcription (C) and expression (D) were analyzed by RT-qPCR and western blot in 22Rv1 cells transfected with SMYD3 small interfering RNAs (siRNAs), control siRNA, pc-vector, or pc-SMYD3 vector respectively. (E) Quantitative results are illustrated for panel (D). (F) 22Rv1 cells and LNCaP cells were transfected with SMYD3-cDNAs plasmid (pc-SMYD3) or control plasmid (pc-vector) for 48 h, followed by treatment of 6 mg/ml huaier extract for another 16 h, then the protein levels of AR-FL and AR-V7 were analyzed by western blot. (G) Quantitative results are illustrated for panel (F). *p < 0.05, **p < 0.01, ***p < 0.001.