Table 1.
Bacterial strains and plasmids used in this study
| Strains /Plasmid | Major characteristics | References |
|---|---|---|
| S. mutans UA159 | wild-type | |
| S. mutans TW406 | UA159/Δsfp, Kanr | This study |
| S. mutans TW406e | UA159/Δsfp, Ermr | This study |
| S. mutans TW406Cm | UA159/Δsfp/pDL278::Psfp, Kanr, Spcr | This study |
| S. mutans TW406Cs | UA159/Δsfp/gtfA::Psfp, Kanr, Spcr | This study |
| S. mutans TW445 | UA159/ΔbacA2, Ermr | This study |
| S. mutans TW405 | UA159/ΔbacC, Kanr | This study |
| S. mutans TW407 | UA159/ΔbacA, Kanr | This study |
| S. mutans TW426 | UA159/ΔcovR, Kanr | This study |
| S. mutans Mub | UA159/ΔTnSmu2, Kanr | (Wu et al., 2010) |
| S. mutans SMU20 | Clinic isolate, sfp+, bacA | (Palmer et al., 2013) |
| S. mutans SMU56 | Clinic isolate, sfp+, sfp+, bacA+ | (Palmer et al., 2013) |
| S. mutans SMU57 | Clinic isolate, sfp+, sfp+, nrpS+, bacA | (Palmer et al., 2013) |
| S. mutans SMU69 | Clinic isolate, sfp+, nrpS+, bacA | (Palmer et al., 2013) |
| S. mutans 27-3 | Clinic isolate, sfp+, bacA+ | This study |
| S. mutans ΔPdeA | UA159/ΔpdeA, Kanr | (Peng, Zhang, et al., 2016) |
| S. mutans ΔDac | UA159/Δdac, Ermr | (Peng, Michalek, et al., 2016) |
| S. mutans ΔDac/ΔPdeA | UA159/ΔpdeA/Δdac, Kanr, Ermr | (Peng, Michalek, et al., 2016; Peng, Zhang, et al., 2016) |
| pDL278 | Shuttle vector, Spcr | (LeBanc & Lee, 1991) |
| pBGK3 | Integration vector, Kanr | (De et al., 2017) |
| pBGS | Integration vector, Spcr | This study |
| E. coli DH10B | Cloning host, mcrA, mcrBC, mrr, and hsd | Invitrogen, Inc. |
Note: Kanr, Ermr and Spcr for kanamycin, erythromycin and spectinomycin resistance, respectively.