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. 2021 Mar 8;11:5442. doi: 10.1038/s41598-021-84828-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Timeline expression of UCP1, ITA7, Lama4 and adiponectin mRNA levels during beige differentiation of hASCs. (A) Representative phase microscopy picture and (B) fluorescent image counterstained with BODIPY, of hASCs at 12 days of differentiation. mRNAs levels of (C) UCP1, (D) ITA7, (E) adiponectin and (F) LAMA4 were evaluated in hASCs relative to D0 (undifferentiated cells) at 8 (D8), 10 (D10) and 12 (D12) days of beige differentiation.UCP1 increases with time with a significant increase at D12. At D12 ITA7 and LAMA4 had a significant decrease compared with previous days (D8 and D10). Adiponectin significantly increases between D0 and D8. Values are means ± standard errors, from one-way ANOVA analysis followed with Tukey's multiple comparisons test. **p < 0.01; ****p < 0.0001; n = 2 different experiments with 4 replicates per group per experiment.