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. 2021 Mar 8;11:5412. doi: 10.1038/s41598-021-84887-2

Figure 2.

Figure 2

Generation of Slc1a3-2A-CreERT2 mice in 129S4 background. (A) In situ hybridization for Gja1 and Slc1a3 of a 10 months old mouse brain. Note disparate expression pattern in the cerebellum, where Slc1a3 is expressed almost exclusively in Bergman glia. (B) Schematic of Slc1a3-2A-CreERT2 targeted locus (elements are to scale). (C–F) Genotyping PCR. PCRs with primers flanking 5; homology region (C), 3′ homology region (D), flanking the whole targeted locus (we never managed to obtain the targeted band for heterozygote, probably due to WT target preference of the assay), (E), and routine genotyping assay (F) were shown. (G) Immunoblot using an anti-Slc1a3 antibody showing Slc1a3 protein levels in WT, heterozygous and homologous knock-in mice. PCR oligo sequences are in Supplementary Table S1 and uncropped images used to prepare panels C-G are shown in Supplementary Fig. S2.