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. 2021 Mar 8;11:5412. doi: 10.1038/s41598-021-84887-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence staining of Slc1a3-CreERT2::RiboTag mice. Representative images from hippocampus and cortex of 7 weeks old mice. RiboTag is a Cre reporter line that upon recombination leads to the expression of an HA (hemagglutinin)-tagged ribosome protein. For each marker, co-staining with anti-HA antibody (HA; RiboTag, red) was used to mark recombined cells; S100β calcium binding protein (astroglial marker), GFAP glial fibrillary acidic protein (astroglial marker), Sox9 SRY-Box Transcription Factor 9 (astroglial marker), Iba1 ionized calcium binding adaptor molecule (microglial marker), Osp oligodendrocyte surface protein, NeuN neuronal nuclei. Scalebar = 50 μm.