Figure 4.

Palmitic acid promoted resistin action. Human SH-Y5Y cells were pretreated with palmitic acid (panel A) or DHA (panel B) and then stimulated by resistin. Phosphorylated Akt and p38 MAP kinase was determined by Western blot using adequate antibodies. (Panel C) cells were treated with DHA or placebo and p38 MAP kinase phosphorylation was determined by Western blot using adequate antibodies. The presented blots in (panel A), (panel B) and (panel C) are from the same membranes that were successively blotted with different antibodies. (Panel D) human SH-SY5Y neuroblastoma cells stably transfected with NF-κB luciferase reporter gene were treated resistin, resistin + palmitic acid or resistin + DHA, and relative luciferase activity determined. Data were presented as means ± SEM (n = 3), ** and *** denoted significant differences vs control at p < 0.005 and p < 0.001, respectively. (Panel E) Human SH-SY5Y neuroblastoma cells were treated with resistin, resistin + palmitic acid or resistin + DHA, and then IL6 and TNFα expression was determined using adequate primers, and normalized using GAPDH. Data were presented as means ± SEM (n = 3), * and ** denoted significant differences vs control at p < 0.05 and p < 0.005, respectively.