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. 2021 Feb 23;8:650724. doi: 10.3389/fmed.2021.650724

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Copyright © 2021 El Zarif, Alió, Alió del Barrio, De Miguel, Abdul Jawad and Makdissy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microscopy study with HRT3 RCM (Heidelberg). (A) Confocal microscopy of the anterior corneal stroma with case 1, G-1. Notice the few nuclear corneal cell density. (B) Nuclear cell density in the anterior stroma with case 1 at 12 months post-operative. Notice the increment of the cell density. (C) Acellular implanted lamina with case 5, G-2 at 1 month post-operative. (D) Recellularization of the anterior surface of the implanted decellularized lamina in the same case 5 at 12 months postoperative. (E) The posterior surface of the recellularized lamina with case 13, G-3 at 1 month after the operation: the presence of few ADASCs with similar morphology of keratocytes. (F) The posterior surface of the recellularized lamina with case 13 at 12 months after surgery: Observe the abundant number of stromal cells.