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. 2021 Feb 18;24:54–66. doi: 10.1016/j.omtn.2021.02.016

Figure 5.

Figure 5

Kdm5b was a critical epigenetic factor for nuclear reprogramming

(A) Representative images of the 4- and 8-cell-stage embryos and blastocysts stained with anti-H3K4me3, anti-Kdm5b, and DAPI. Scale bar, 20 μm. (B and C) Statistical analysis of Kdm5b and H3K4me3 signal intensity in the 4- and 8-cell- and blastocyst-stage IVF embryos. ∗∗p < 0.01. (D) Representative images of the 8-cell IVF and SCNT embryos stained with anti-H3K4me3. Scale bar, 20 μm. (E) Representative images of the 8-cell IVF and SCNT embryos stained with anti-Kdm5b. Scale bar, 20 μm. (F) Statistical analysis of Kdm5b signal intensity at the 8-cell-stage IVF and SCNT embryos. Student’s t test, ∗∗p < 0.01. (G) Representative images of control and Kdm5b-injected SCNT embryos. Scale bar, 100 μm. (H) Development of control and Kdm5b-injected SCNT embryos. Shown are percentages of embryos that reached the indicated stages. Error bars represent SEM. ∗p < 0.05, ∗∗p < 0.01, Student’s t test.