Figure 6.

Knockdown of lnc_3712 demethylated H3K4me3 via upregulating Kdm5b

(A) Kdm5b staining in lnc_3712 knockdown GFF cells. Scale bar, 20 μm. (B) H3K4me3 staining in lnc_3712 knockdown GFF cells. Scale bar, 20 μm. (C) H3K4me1 staining in lnc_3712 knockdown GFF cells. Scale bar, 20 μm. (D) Statistical analysis of Kdm5b, H3K4me3, and H3K4me1 signal intensity between lnc_3712 knockdown and NC GFF cells. Student’s t test, ∗∗∗∗p < 0.0001. (E) Western blot of Kdm5b in GFF cells that lnc_3712 was knocked down. (F) Statistical analysis of Kdm5b in lnc_3712 knockdown GFF cells. LSD test, statistical differences among groups were labeled with different letters. (G) Representative images of NC and lnc_3712 and Kdm5b double knockdown SCNT embryos. Blastocysts are indicated by the white arrows. Scale bar, 200 μm. (H) Statistical analysis of blastocyst rate in NC and double knockdown SCNT embryos.