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. 2021 Feb 23;12:628879. doi: 10.3389/fmicb.2021.628879

FIGURE 3.

FIGURE 3

TamA and TamB are not required for outer membrane insertion of trimeric autotransporters. (A) Western blot analysis of wild-type HB101 and tamA and tamB mutant outer membrane fractions confirming the loss of each protein in the corresponding mutant using antibodies specific to TamA and B with anti-BamA specific antibodies used as a positive control. (B) Western blot analysis of wild-type HB101 and tamA and tamB mutant outer membrane fractions expressing either empty vector (–) or YadA/SadA (+) using YadA or SadA specific antibodies to confirm the presence of the proteins in the outer membrane.