TABLE 1.

Strains and plasmids used in this study.

Strain or plasmid	Characteristics	References
Strains
E. coli HB101	Laboratory E. coli strain
E. coli HB101 bamB:aph	bamB gene replaced with a kanamycin cassette	Rossiter et al., 2011
E. coli HB101 bamC:aph	bamC gene replaced with a kanamycin cassette	Rossiter et al., 2011
E. coli HB101 bamE:aph	bamE gene replaced with a kanamycin cassette	Rossiter et al., 2011
E. coli BW25113	K-12 derivative	Baba et al., 2006
E. coli BW25113 tamA:aph	tamA gene replaced by kanamycin cassette	This study
E. coli BW25113 tamB:aph	tamB gene replaced by kanamycin cassette	This study
E. coli JWD3	E. coli K-12 BamA depletion strain.	Lehr et al., 2010
E. coli JCM290	E. coli K-12 BamD depletion strain.	Malinverni et al., 2006
Plasmids
pQE60 NdeI	pQE60 with added NdeI restriction site in the multiple-cloning site and previously existing NdeI site removed	Raghunathan et al., 2011
pDR03	pQE60NdeI carrying sadA gene in the NdeI-HindIII site	Raghunathan et al., 2011
pET22b(+)	IPTG inducible vector	Invitrogen
pET26b(+)	IPTG inducible vector	Invitrogen
pDR01	sadA gene in the NdeI-EcoRI sites of pET22b(+)	Raghunathan et al., 2011
pET26b-SadA	sadA gene in the NdeI-EcoRI sites of pET26b(+)	This study
pIBA2C	Anhydrotetracycline inducible vector.	IBA Lifesciences
pIBA2-YadA	yadA gene in AHTC-inducible expression vector pASK-IBA2	Grosskinsky et al., 2007
pIBA2C-YadA	yadA gene in the XbaI-SalI sites of pASK-IBA2C	This study