Figure 3.

LSD1 promotes prostate cancer cell survival by destabilizing FBXW7 at post-translational level. (A) The relative cell number of LNCaP and PC3 cell lines was detected by cell counter after transfected with SiNTC or SiLSD1. (B) Representative bands of Western blot analysis for the expression of LSD1, FBXW7, c-MYC, and NOTCH-1 in indicated groups. (C, D) Relative expression level of each protein in (B) (SiNTC vs. SiLSD1). (E) Western Blot analysis for the protein expression of LSD1 and FBXW7 in rescue assay. (F) Relative cell number of indicated groups in rescue assay. Values are expressed as the mean ± SEM.*P < 0.05, **P<0.01, ***P<0.001, relative to the SiNTC group, n = 3. Protein levels were normalized by GADPH.