FIGURE 3.

HSV-1-induced Arc is enriched in neuronal somata rather than dendrites of infected neurons. (A) Immunocytochemical analyses of Arc protein subcellular distribution during infection kinetics. Middle and low panels show magnifications of somata and dendrites. Scale bars: 20 μm (B) Quantification of Arc fluorescence intensity in HSV-1 infected neurons versus positive (BDNF) and negative (Mock) controls. Statistical significance was determined by One-Way ANOVA, followed by Tukey’s test. Bars represent the mean ± SD of biological replicates (10 neurons per treatment). Different letters above the mean bars apply to significant differences between groups p < 0.05. The images and quantifications are representative of three independent experiments.