Antibodies |
Rabbit polyclonal anti-Arc |
Synaptic Systems |
Cat# 156003 |
Mouse monoclonal anti-ICP8 |
Santa Cruz Biotechnology |
Cat# sc-53329, clone10A3 |
Mouse monoclonal anti-Drebrin |
Abcam |
Cat# ab-12350, clone M2F6 |
Mouse monoclonal anti-PSD95 |
Thermo Fisher Scientific |
Cat# MA1-046, clone 7E3-1B8 |
Mouse monoclonal anti-CaMKIIβ |
Thermo Fisher Scientific |
Cat# 139800, clone CB-beta1 |
Rabbit polyclonal anti-phospho-CaMKIIβ/γ/δ (Thr287) |
Thermo Fisher Scientific |
Cat# PA5-39731 |
Rabbit polyclonal anti-Total Erk1/2 |
Cell Signaling Technology |
Cat# 4695S |
Rabbit polyclonal anti-phospho Erk1/2(MAPKpT202/pY204 |
Cell Signaling Technology |
Cat# 9101S |
Rabbit monoclonal anti-CREB |
Cell Signaling Technology |
Cat# 9197, clone 48H2 |
Rabbit monoclonal anti-phospho CREB Ser133 |
Cell Signaling Technology |
Cat# 9198, clone 87G3 |
Rabbit polyclonal anti-HSV-1/2 total proteins |
Abcam |
Cat# ab9533 |
Mouse monoclonal anti-GAPDH |
Thermo Fisher Scientific |
Cat# MA5-15738, clone GA1R |
Mouse monoclonal anti-β-Actin |
Sigma Aldrich |
Cat# A5441, clone AC-15 |
Chicken polyclonal anti-MAP-2 |
Encor Biotechnology |
Cat# CPCA-MAP2 |
Alexa-488, Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488. |
Thermo Fisher Scientific |
Cat# A32723 |
Alexa-568, Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568. |
Thermo Fisher Scientific |
Cat# A-21090 |
Bacterial and Virus Strains |
HSV-1 (strain F) |
supplied by Dr. Bernard Roizman, Northwestern University, Chicago, United States. |
|
Chemical, Peptides and Recombinant Proteins |
Trypsin-EDTA (0.05%), phenol red |
Thermoscientific |
Cat# 25300054 |
Poly-L-lysine hydrobromide |
Sigma Aldrich |
Cat# P2636-500MG |
MEM medium |
Thermoscientific |
Cat# 11095080 |
Fetal bovine serum |
Sigma Aldrich |
Cat# 1 F7524 |
D-Glucose |
Sigma Aldrich |
Cat# G8769 |
Sodium Pyruvate (100 mM) |
Thermo Fisher Scientific |
Cat# 11360070 |
Neurobasal medium |
Thermo Fisher Scientific |
Cat# 21103049 |
GibcoTM B-27TM Supplement, Serum Free, 10 mL |
Thermo Fisher Scientific |
Cat# 11530536 |
GlutaMAX |
Life Technologies |
Cat# 35050-038 |
BDNF |
Alomone |
Cat# B-250 |
Tetrodotoxin citrate, Na+ channel blocker |
Abcam |
Cat# ab120055 |
U0126 monoethanolate |
Sigma Aldrich |
Cat# U120 |
Triton X-100 |
Sigma Aldrich |
Cat# T8787 |
PierceTM 16% Formaldehyde (w/v), Methanol-free |
Thermo Fisher Scientific |
Cat# 11586711 |
Phalloidin |
Thermo Fisher Scientific |
Cat# 10656353 |
RIPA buffer 1X |
Sigma Aldrich |
Cat# R0278 |
Protease and Phosphatase inhibitors |
Sigma Aldrich |
Cat# 11836170001 |