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. 2021 Feb;9(3):245. doi: 10.21037/atm-20-4707

Figure 3.

Figure 3

Maturation and characterization of human iRPE cells. (A) Scheme of iRPE cell culture and characterization methods. Phase-contrast images of human iRPE cells at 2 days (B), 8 days (C), 16 days (D) and 25 days (E) in culture. Magnified images are appended in the top right corners. Scale bars, 200 µm (B), 100 µm (C-E). Representative immunofluorescence images of iRPE cells. iRPE cells expressed typical RPE markers, RPE65 (G), ZO-1 (H) and MiTF (L). (J) Magnified view of inset displays localization of RPE65 and ZO-1 to RPE cells. Cell nuclei (blue) were stained with DAPI (F, K), and merged images are shown in the panels (I, M). Scale bars, 25 µm.