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. 2021 Feb;9(3):232. doi: 10.21037/atm-20-3399

Figure 4.

Figure 4

MiR-4729 was expressed at low levels in hemorrhoids, and targets METTL14. (A) The bioinformatics analysis showed that there were 10 microRNAs that could potentially regulate METTL14 expression. (B) miR-4729 expression was significantly decreased in hemorrhoids. **P<0.01 vs. healthy tissue; t-test; n=8. (C) Mature miR-4729 and the 7 bases at specific METTL14 sites can be completely complementary and paired. (D) The luciferase reporter assay analysis showed that wild type (WT) miR-4729 overexpression significantly decreased luciferase expression carrying WT Mettl14. Other combinations did not affect the level of luciferase expression. *P<0.05 vs. psiCHECK2; t-test; n=4. (E) The quantitative real-time PCR (qPCR) results showed that the level of Mettl14 mRNA expression in miR-4729-HUVECs was significantly lower than that of the control group (miR-mut-HUVECs). **P<0.01 vs. miR-mut-HUVECs; t-test; n=3. (F) The western blot results showed that the level of METTL14 protein expression in miR-4729-HUVECs was significantly lower than that of the miR-mut control cells. (G) Immunofluorescence staining showed that the level of METTL14 protein expression in the miR-4729-HUVECs was significantly lower than that in the miR-mut-HUVECs. Magnification: 400×.