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. 2021 Feb;9(3):232. doi: 10.21037/atm-20-3399

Figure 7.

Figure 7

MiR-4729 overexpression inhibits the molecular loop of the TIE1/VEGFA signaling pathway in vascular endothelial cells. The interaction networks with TIE1 and angiogenesis related proteins were identified using (A) bioinformatics tools. (B) The qPCR results showed that the levels of TIE1, VEGFA, VEGFR2, vWF, and CD31 and other gene expression in the miR-4729-HUVEC group were significantly lower than that of the miR-mut-HUVEC group. **P<0.01 vs. miR-mut-HUVECs; t-test; n=3. (C) The western blotting results showed that the levels of TIE1, VEGFA, VEGFR2, vWF, CD31, and other protein expression in the miR-4729-HUVEC group were significantly lower than that of the miR-mut-HUVEC group. (D) MiR-4729 weakens the m6A modification of the 3'UTR of TIE1mRNA by inhibiting METTL14, inhibiting the expression of TIE1/VEGFA signaling molecules in vascular endothelial cells, the proliferation of vascular endothelial cells, and the vascular proliferation mechanism.