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. 2021 Feb;9(3):234. doi: 10.21037/atm-19-4738

Figure 5.

Figure 5

Glucose affected cell adhesion by activation of the PI3K/AKT/NF-κB signaling pathway on GDM-HUVECs via Cx43. (A) Expression levels of P-Akt, p-IκBα, and p-p65 protein expression changed according to the alteration of glucose levels. Data are presented as mean ± SEM; n=5, *, P<0.05 vs. N; #, P<0.05 vs. GDM. (B) P-Akt, p-IκBα, and p-p65 protein expression were also attenuated when specific Cx43-siRNA (50 nM, 48 h) was added in GDM-HUVECs. Data are presented as mean ± SEM; n=5, *, P<0.05 vs. control; #, P<0.05 vs. GDM. The corresponding proteins were not affected. Histograms were made according to the ratio of phosphorylated protein/total protein. Cx43, Connexin43; HUVECs, human umbilical vein endothelial cells; GDM, gestational diabetes mellitus; NC, negative control.