Figure 1.

OPP labeling as a method to evaluate protein biosynthesis in islet cells. (A) Transmitted light image of reaggregated islet cells (24 h after islet cell isolation) with a 4’,6-diamidino-2-phenylindole staining and a second image displaying the different rates of OPP labeling. The bar represents 10 µm. (B) Quantification of OPP labeling of rat islet cells in 3 different experiments obtained with or without OPP (autofluorescence) and with OPP supplemented with cycloheximide (cyclo). *P < 0.05 and **P < 0.01.