Figure 2.
Energy deficit in sensory neurons from diabetic rats was prevented by IGF-1 treatment. DRG neurons derived from adult control or STZ-induced diabetic rats were transfected with AT1.03YEMK (medium affinity) for 48 h. A subgroup of DRG neurons from diabetic rats were treated with 10 nM IGF-1 for the final 24 h. (A, B) Cell body and (C, D) axonal images were taken using confocal microscope and FRET efficiency (YFP/CFP ratio) was calculated for each group of ATP sensors. In (E), FRET efficiency of cell body and neurites of the neurons are compared within groups. In (F), FRET efficiency of proximal and distal parts of the longest neurites are compared within groups. Data are mean ± SEM of N > 30 images for cell bodies and N > 8 for axons (1–2 cell bodies/neurites per image were analysed); ∗ = p < 0.05 or ∗∗ = p < 0.01 or ∗∗∗∗ = p < 0.0001; analysed by Student's t-test or one-way ANOVA with Tukey's post-hoc test.