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. 2021 Feb 16;24(3):102197. doi: 10.1016/j.isci.2021.102197

Figure 3.

Figure 3

MATR3 downregulation affects the expression levels of pluripotency determinants

(A and B) WB and qRT-PCR analyses for OCT4, NANOG, KLF4, and LIN28A performed on shMATR3-and shCtrl-hiPSC cultures. WB and RT-qPCR results were normalized on the internal housekeeping (β-ACTIN) and reported in comparison to the shCtrl samples (reported as value 1 in the graph). Both analyses were presented as the mean ± SEM of three biological replicates. p value was calculated by one-way ANOVA (∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001).

(C and D) WB and RT-qPCR analyses for OCT4, NANOG, KLF4, and LIN28A performed on shCtrl-, shMATR3- (−), shMATR3- and on MATR3 rescued shMATR3- (+) cultures. WB and qRT-qPCR analyses were normalized on the internal housekeeping (β-ACTIN) and reported in comparison to the shCtrl values (reported as value 1 in the graph). Values as reported as mean ± SEM. p value was calculated by one-way ANOVA (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001).