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. 2021 Feb 16;24(3):102197. doi: 10.1016/j.isci.2021.102197

Figure 6.

Figure 6

MATR3 stabilizes NANOG and LIN28A mRNAs

(A) MATR3 RNA immunoprecipitation (RIP) performed on parental hiPSCs followed by qRT-PCR analysis. Fold enrichment was relative to IgG. Values are presented graphically as mean ± SEM from three biological replicates. One-way ANOVA (∗∗∗∗p < 0.0001).

(B) qRT-PCR analysis for the evaluation of mRNA half-lives in shCtrl and shMATR3 hiPSCs performed by actinomycin-D assay. qRT-PCR values are normalized to their internal housekeeping (β-ACTIN). Results are expressed as percentages of mRNA abundance relative to Time 0. Statistical analysis was performed on the mean of three biological replicates, two-way ANOVA (∗∗∗p < 0.001).